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Weiren Huang

Vice-Dean of the Translational Medicine Research Institute of Shenzhen, Researcher, Doctoral Supervisor, and Master's Supervisor.

PI介紹

Researcher, Outstanding Talent of the Guangdong Province "Special Support Program" (Nanyue Bai Jie), National-Level High-Level Leading Talent of Shenzhen, Chief Scientist of the National Key R&D Program, mainly engaged in clinical and applied basic research on urological tumors. Currently, he is the Director of the Discipline Construction Office at Shenzhen Second People’s Hospital, Vice Dean of the School of Translational Medicine, Executive Director of the National and Local Joint Cancer Genomics Clinical Application Key Technology Engineering Laboratory, Deputy Director of the National and Local Joint Medical Synthetic Biology Application Key Technology Engineering Laboratory, Deputy Director of the Guangdong Provincial Key Laboratory of Urological and Reproductive Cancer System and Synthetic Biology, Director of the Shenzhen Key Laboratory of Medical Gene Reprogramming Technology, Vice Chairman of the Synthetic Biology Branch of the China Biopharmaceutical Technology Association, Member of the Urology Branch of the Chinese Medical Association, Member of the Synthetic Biology Committee of the Chinese Society of Biotechnology, and Vice President of the Shenzhen Synthetic Biology Association.

Focusing on the clinical needs of urological tumors, his team continuously strengthens interdisciplinary integration. By combining tumor precision diagnosis and treatment with synthetic biology research, they have developed a tumor high-fidelity 3D organoid construction and application platform using clinical sample-derived tissues, which simulates the real in vivo environment of urological tumors. This model is used to explore precise drug screening, resistance molecular mechanisms, artificial gene circuit synthesis, and engineered immune cell functional evaluation. It provides an efficient in vitro model for functional testing in precision medicine. Focusing on tumor synthetic biology enabling technologies and common scientific issues, they have developed new clinical treatment methods for bladder cancer, conducting research on bladder cancer-specific identification and diagnosis and treatment. By integrating different biological signals, including gene mutations, methylation, and promoter abnormalities, they design artificial gene circuits to accurately distinguish cancer cells from normal cells, reprogram tumor cell fate, and directly kill them. They also develop theoretical foundations for tumor gene circuit design and establish an "integrated" targeted delivery system that matches different types of gene circuits to provide optimal carriers for efficient targeted engineering.

His research provides a rich toolbox for precise identification and intervention in urological tumors and has developed related technical theories, providing an important scientific basis for precision treatment. His work has been published in over 100 international journals, including Nature Methods, Nature Communications, Advanced Science, Genome Biology, ACS Applied Materials & Interfaces, and Clinical and Translational Medicine, receiving high praise from domestic and international peers. As the first author, he has received the China Medical Science and Technology Award and the Shenzhen Natural Science Award and has been granted 14 national invention patents. The development of this research has enriched the theoretical and scientific foundations of tumor synthetic biology technology, improved the precision medicine system for tumors, and established a "design, test, application" closed-loop, which will promote the transition of gene circuit-based tumor synthetic biology therapies from proof-of-concept to clinical application.


教育背景

Education:

1999/09–2003/07: Bachelor's Degree in Biology, Hunan Normal University

2004/09–2006/07: Master's      Degree in Physiology, Sun Yat-sen University

2006/09–2009/07: Ph.D. in      Physiology, Sun Yat-sen University


工作經歷

Work Experience:

2013/09–Present: Director  of the Discipline Construction Office, Deputy Director of the Central  Laboratory, Researcher, Shenzhen Second People’s Hospital

2021/03–Present: Executive Vice Dean and Full-time Principal Investigator, Translational  Medicine Research Institute, Shenzhen Second People’s Hospital

2009/10–2012/11: Postdoctoral Researcher, Peking University Shenzhen-Hong Kong University  of Science and Technology Medical Center, Peking University School of Medicine


研究方向和興趣

Research Directions and Interests:

Tumor Synthetic Biology  Research

Tumor Organoid Culture  and Precision Medicine Research

Development of Gene  Editing Technologies

Development of Next-Generation Molecular Diagnostics Technologies


科研項目

Research Projects:

National Key R&D Program(2019YFA0906000)
Title: Mechanisms of Cell Microenvironment Remodeling and Disease      Treatment Strategies Based on Gene Circuit
Duration: Dec 2019 – Dec 2024
Funding: 18.53 million RMB
Status: Ongoing, Principal Investigator

National Natural Science Foundation of China, General Program(81772737)
Title: Screening and Mechanism Study of Bladder Cancer      Chemoresistance-Associated Gene MED7
Duration: Jan 2018 – Dec 2021
Funding: 900,000 RMB
Status: Completed, Principal Investigator

Shenzhen Key Laboratory Project(ZDSYS201504301722174)
Title: Shenzhen Gene Reprogramming Technology Key Laboratory
Duration: Sept 2015 – Sept 2017
Funding: 3 million RMB
Status: Completed, Principal Investigator

Shenzhen Science and Technology Plan Basic Research Project(JCYJ20170413161749433, Base 20170288)
Title: Application and Research of Gene Editing Technology in      Disease Treatment
Duration: Jun 2017 – Jun 2020
Funding: 2.5 million RMB
Status: Completed, Principal Investigator

Shenzhen Science and Technology Innovation Plan Project – Basic      Research (Key Project)(JCYJ20200109120016553,      Base 2020N145)
Title: High-Throughput Screening Platform for Personalized      Anticancer Drugs Based on Tumor Organoid Chips
Duration: Nov 2020 – Nov 2023
Funding: 2 million RMB
Status: Ongoing, Principal Investigator

Shenzhen Science and Technology Plan International Cooperation      Research Project(GJHZ20180926165202081)
Title: Subclonal Heterogeneity of Bladder Tumors and Its Role in      Evolution and Metastasis
Duration: Sept 2019 – Sept 2021
Funding: 500,000 RMB
Status: Ongoing, Principal Investigator


代表性成果

Thesis

1.Liu Y.,Zhan Y.,Chen Z.,He A.,Li J.,Wu H.,Liu L.,Zhuang C.,Lin J.,Guo X.,Zhang Q.,Huang W.*,Cai Z*..Directing cellular information flow via CRISPR signal conductors.NATURE METHODS.2016, 13 (11) :938-944.

2.Li Z, Xu H, Yu L, Wang J, Meng Q, Mei H, Cai Z, Chen W, Huang W. Patient-derived renal cell carcinoma organoids for personalized cancer therapy.[J]. Clinical and translational medicine,2022,12(7).

3.Li Z, Xu H, Gong Y, Chen W, Zhan Y, Yu L, Sun Y, Li A, He S, Guan B, Wu Y, Xiong G, Fang D, He Y, Tang Q, Yao L, Hu Z, Mei H, He Z, Cai Z, Guo Y, Li X, Zhou L, Huang W. Patient-Derived Upper Tract Urothelial Carcinoma Organoids as a Platform for Drug Screening. Advanced Science. 2021 Dec 16:e2103999.

4. Ma Y.#,Mao G.#,Huang W.#,Wu G.,Yin W.,Ji X.,Deng Z.,Cai Z.,Zhang X.,He Z.*,Cui Z.*.Quantum Dot Nanobeacons for Single RNA Labeling and Imaging.Journal of the American Chemical Society.2019.141(34):13454-13458.

5.Zhan H.#,Zhou Q#,Gao Q.#,Li J.#,Huang W.*,Liu Y.*.Multiplexed promoterless gene expression with CRISPReader.Genome Biology.2019,20:113.

6.Wang Z.#,Li K.#,Chen W.#,Wang X.,Huang Y.,Wang W.,Wu W.,Cai Z.*,Huang W.*.Modulation of SRSF2 expression reverses the exhaustion of TILs via the epigenetic regulation of immune checkpoint molecules.Cellular and molecular life sciences .2020;77(17):3441–3452.

7.Ma Y, Mao G, Wu G, Cui Z, Zhang XE, Huang W. .CRISPR-dCas9-Guided and Telomerase-Responsive Nanosystem for Precise Anti-Cancer Drug Delivery.ACS APPLIED MATERIALS & INTERFACES.2021;13(7):7890-7896.

8.Wang J, Feng X, Li Z, Chen Y, Huang W*. .Patient-derived  organoids as a model for tumor research.Progress in Molecular Biology and Translational Science.22;189(1):259-326.

9.Fan J.#*,Liu Y.#,Liu Lisa,Huang Y., Li X.,Huang W.*.A Multifunction Lipid-Based CRISPR-Cas13a Genetic Circuit Delivery System for Bladder Cancer Gene Therapy.ACS Synthetic Biology.2020,9(2):343-355.

10.Ma Y.#*, Mao G.#,Wu G.#,Fan J.,He Z.,Huang W.*.A novel nano-beacon based on DNA functionalized QDs for intracellular telomerase activity monitoring.SENSORS AND ACTUATORS B-CHEMICAL.2020,304:127385.

11.Xie H.#,Zhan H.#,Gao Q.#,Li J.#,Zhou Q,Chen Z.,Liu Y.,Ding M.,Xiao H.,Liu Y.*,Huang W.*,Cai Z.*.Synthetic artificial "long non-coding RNAs" targeting oncogenic microRNAs and transcriptional factors inhibit malignant phenotypes of bladder cancer cells.CANCER LETTERS.2018,9(3):209;94-106.


Patent Grants

Huang Weiren, Liu Yuchen,      Zhan Yonghao, Cai Zhiming. "Tetracycline-Inducible Artificial      MicroRNA Element," ZL 2015 1 0690643.8, granted on July 1, 2021, Invention      Patent.

Huang Weiren, Chen      Zhichong, Liu Yuchen, Cai Zhiming. "Theophylline-Inducible RNA      Interference Switch System and Its Gene Regulation Method and      Application," ZL 2016 1 0741107.0, granted on February 21, 2020, Invention      Patent.

Huang Weiren, Cai      Zhiming, Lin Fan. "Light-Controlled Gene Device for Efficient      Regulation of Tumor Cell Phenotype," ZL 2016 1 0795393.9, granted on      December 17, 2019, Invention Patent.

Huang Weiren, Liu Yuchen,      Cai Zhiming. "Molecular Genetic Device for Specific Detection of      Bladder Cancer Cells," ZL 2016 1 0892544.2, granted on September 17,      2019, Invention Patent.

Huang Weiren, Liu Yuchen,      Cai Zhiming, Xu Wen, Chen Zhichong. "asRNA Targeting the MALAT-1 Gene      and Its Applications," ZL 2016 1 0321374.2, granted on May 13, 2019, Invention      Patent.

Huang Weiren, Liu Yuchen,      Cai Zhiming, Chen Zhichong. "CRISPR-Cas9-Based Gene Regulation Device      and Gene Regulation Method," ZL 2016 1 0741981.4, granted on      September 13, 2019, Invention Patent.

Huang Weiren, Liu Yuchen,      Cai Zhiming. "Nucleic Acid Aptamer for Human NF-kB and Its      Applications," ZL 2014 1 0208520.1, granted on June 5, 2018, Invention      Patent.

Huang Weiren, Liu Yuchen,      Cai Zhiming. "Method for Constructing a Molecular Genetic Device for      Specific Detection of Bladder Cancer Cells," ZL 2014 1 0441470.1,      granted on January 4, 2017, Invention Patent.

Ma Yingxin, Huang Weiren.      "Telomerase Activity Detection Kit and Detection Method," ZL      2018 1 0503919.0, granted on June 15, 2021, Invention Patent.

Liu Yuchen, Huang Weiren,      Cai Zhiming. "Nucleic Acid Aptamer for Human β-Catenin and Its      Applications," ZL 2014 1 0841057.4, granted on March 2, 2018, Invention      Patent.


學術榮譽

Academic Honors:

2020: China Medical Science and Technology Award, Youth Science and Technology Award

2019: Guangdong Medical Science and Technology Award, First Prize

2018: China  Industry-University-Research Cooperation Innovation Award

2017: Shenzhen Natural  Science Award, Second Prize

2017: China Science Outstanding Young Scientist Award

2014: China Medical Science and Technology Award, Second Prize


學術兼職

Academic Positions:

2019–Present: Vice Chairman, Low-Carbon Medicine Committee, Shenzhen Medical Association

2019–Present: Vice Chairman, Synthetic Biology Technology Committee, China Pharmaceutical Biotechnology  Association

2018–Present: Vice President, Shenzhen Synthetic Biology Association

2018–Present: Member, Hereditary Tumor Committee, Guangdong Anti-Cancer Association

2018–Present: Member, Basic ScienceGroup, Urology Branch, Chinese Medical Association

2018–Present: Member, Andrology Committee, Shenzhen Medical Association

2018–Present: Member, Synthetic Biology Committee, China Society of Biotechnology